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Digital image processing of crystalline specimens examined by electron microscopy
1Department of Electrical Engineering, Kogakuin University, Tokyo, Japan.
Journal of Electron Microscopy Technique
|December 1, 1988
Summary
Digital processing of electron microscope images aids in analyzing crystalline structures. This technique helps visualize atomic and molecular arrangements in materials science and biology, revealing defects and 3D molecular structures.
Area of Science:
- Materials Science
- Biology
- Crystallography
- Digital Image Processing
Background:
- Electron microscopy provides high-resolution images of crystalline specimens.
- Analyzing these images requires advanced digital processing techniques.
- Fourier decomposition and gradient/Laplacian operators are key analytical methods.
Purpose of the Study:
- To discuss principles of structural analysis using digital processing of electron micrographs.
- To review applications of these techniques in Materials Science and Biology.
- To demonstrate the localization of atoms, molecules, and defects, and to explore 3D molecular arrangements.
Main Methods:
- Digital processing of crystalline specimens imaged via electron microscopy.
- Application of Fourier decomposition for structural analysis.
- Utilizing gradient and Laplacian operators for image enhancement.
- Employing helical diffraction theory for 3D molecular structure determination.
Main Results:
- Successful localization of atoms, molecules, and defects in gold, tungsten, and cadmium selenide crystals.
- Determination of the three-dimensional arrangement of molecules in biological samples like alveolar soft part sarcoma, Hirano bodies, and neurofibrillar tangles.
Conclusions:
- Digital processing of electron micrographs is a powerful tool for detailed structural analysis.
- The methods discussed are effective for both materials science and biological applications.
- Advanced digital techniques enable precise visualization of atomic, molecular, and defect structures.