Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent progress in side-chain amino acid-based polymers: synthesis, self-assembly, and emerging biomedical applications.

Journal of materials chemistry. B·2026
Same author

In Situ Biosynthesis of Pd Nanocrystals in Bifidobacterium Bioreactor as Dual Immune Stimulators for Immuno-Chemodynamic Therapy of Cold Tumor.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Carbon-Bridged-Mediated Electron Transfer in Cu<sub>2</sub>O-ZnO Nanocomposites Enabling ROS-Driven Antimicrobial Activity.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Targeting Mitochondrial Oxidative Stress by Calcium/Copper/Elesclomol Tri-Overloaded Nanocages for Osteosarcoma Immunotherapy via Immunogenic Cell Death.

ACS nano·2025
Same author

pH-Responsive carrier-free nanoparticles based on teicoplanin and borneol for enhanced MRSA infectious wound healing.

Biomaterials science·2025
Same author

Eradication of Intracellular <i>Staphylococcus aureus</i> Persisters via On-Site Antibiotic Delivery by Poly(amino acid) Nanoparticles.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: Jul 1, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

11.3K

Adhesive Gold Nanoparticles for Easy and Controlled Surface Coating.

Tianchi Liu1, Fan Yang2, Xing Wang3

  • 1Department of Chemistry and Chemical Biology, Schaefer School of Engineering and Science , Stevens Institute of Technology , Hoboken , New Jersey 07030 , United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 24, 2019
PubMed
Summary

Researchers developed adhesive gold nanoparticles (Au NPs) using borated dopamine dithiocarbamate. These nanoparticles enable easy and controllable surface coating on diverse materials via pH-activated catechol reactions.

More Related Videos

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
10:38

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

13.0K
Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting
05:51

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting

Published on: February 26, 2019

6.0K

Related Experiment Videos

Last Updated: Jul 1, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

11.3K
Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
10:38

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

13.0K
Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting
05:51

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting

Published on: February 26, 2019

6.0K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Surface Chemistry

Background:

  • Gold nanoparticles (Au NPs) are crucial nanomaterials with unique properties and diverse applications.
  • Decorating universal surfaces with Au NPs is a significant challenge in materials science.
  • Developing novel methods for controlled surface functionalization is highly desired.

Purpose of the Study:

  • To develop a novel method for creating adhesive gold nanoparticles.
  • To achieve spontaneous and controllable surface coating of gold nanoparticles on various materials.
  • To explore the potential of catechol-mediated reactions for surface functionalization.

Main Methods:

  • Functionalization of gold nanoparticles with borated dopamine dithiocarbamate.
  • Activation of functionalized gold nanoparticles at acidic pH.
  • Surface coating initiated by deprotected catechol-mediated reactions.
  • Demonstration of surface coating on materials with diverse chemical and physical properties.

Main Results:

  • The synthesized gold nanoparticles are nonreactive in colloidal solution but become adhesive upon activation at acidic pH.
  • A facile and controllable surface coating process was achieved on various substrates.
  • The high reactivity of the catechol group facilitated spontaneous surface deposition.
  • The method demonstrated broad applicability across different material types.

Conclusions:

  • Adhesive gold nanoparticles functionalized by borated dopamine dithiocarbamate offer a new approach for surface coating.
  • The pH-triggered catechol-mediated reaction provides a versatile and efficient surface modification strategy.
  • This method holds significant potential for applications requiring controlled nanoparticle deposition on diverse surfaces.