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Updated: Jan 30, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
Published on: March 14, 2014
MiR-30a-5p frequently downregulated in prostate cancer inhibits cell proliferation via targeting PCLAF
Hu Zhao1,2, Xiaofeng Lai3, Wei Zhang4
1a Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou Dongfang Hospital of Xiamen University (900 Hospital of the Joint Logistics Team), Fuzhou, Fujian, P.R. China.
Abstract:
MicroRNAs (miRNAs) have vastly expanded our view of RNA world in intracellular signal regulating networks. Here, we functionally characterized a normally highly expressed miRNA, miR-30a-5p (MIMAT0000087), which exhibits downregulated expression profiles in prostate cancer samples. MiR-30a-5p knockdown and overexpression in PC-3 cell line alters cell proliferation supporting a tumour suppressor role. We also discovered that PCLAF is the direct target of miR-30a-5p. Notably, PC-3 cell proliferation is inhibited by miR-30a-5p/PCLAF axis. miR-30a-5p represents a novel molecule of functionally important miRNAs which may shed light on the novel therapeutic targets for prostate cancer.
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