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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Long non-coding RNA PSMB8-AS1 regulates influenza virus replication.

Sunil More1,2, Zhengyu Zhu1,2, Kong Lin1,2

  • 1a Oklahoma Center for Respiratory and Infectious Diseases , Oklahoma State University , Stillwater , OK , USA.

RNA Biology
|January 24, 2019
PubMed
Summary

Long non-coding RNAs (lncRNAs) regulate gene expression and antiviral activity. This study identified lncRNA PSMB8-AS1 as a potential target for developing new influenza virus antiviral therapies.

Keywords:
Long non-coding RNAs (lncRNAs)PSMB8-AS1anti-viral activity

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Area of Science:

  • Molecular Biology
  • Virology
  • Genomics

Background:

  • Long non-coding RNAs (lncRNAs) are emerging regulators of gene expression.
  • Limited research exists on lncRNAs' role in influenza virus infection and antiviral defense.

Purpose of the Study:

  • To identify and characterize lncRNAs involved in influenza virus replication.
  • To explore the potential of lncRNAs as therapeutic targets against influenza.

Main Methods:

  • RNA sequencing was used to analyze lncRNA expression in influenza-infected human lung epithelial cells (A549).
  • Gene ontology analysis was performed on neighboring genes of differentially expressed lncRNAs.
  • Real-time PCR validated selected lncRNAs.
  • CRISPR interference was used to repress PSMB8-AS1 expression.

Main Results:

  • 1,912 lncRNAs showed significant changes during influenza A infection.
  • Enriched gene ontology terms included type I interferon signaling and cellular response.
  • Seven lncRNAs were validated and found to be induced by multiple influenza strains and interferon-β1.
  • Repressing lncRNA PSMB8-AS1 reduced viral replication markers.

Conclusions:

  • lncRNAs are significantly altered during influenza virus infection.
  • lncRNA PSMB8-AS1 plays a role in influenza virus replication.
  • PSMB8-AS1 represents a potential host factor target for novel antiviral therapies against influenza.