Piriform cortex brain slices: techniques for isolation of synaptic inputs

Methods are described for preparation of 3 different slices of piriform cortex which allow convenient study of pyramidal neurons and segregation of synaptic inputs. In slices cut parallel to the pyramidal neurons (perpendicular to the brain surface) one can study chemosensitivity of the various parts of the dendritic tree and the soma. By selected division of this slice the population postsynaptic response to activation of the lateral olfactory tract can be studied without action potential generation. Alternatively the superficial lateral olfactory tract can be removed. Stimulation of deeper regions of the slice under these circumstances elicits a pharmacologically different excitation which appears to be that of association fibers.