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Complete genome sequence of an alternavirus from the phytopathogenic fungus Fusarium incarnatum

Xiaoting Zhang1, Yuan Xie1, Fang Zhang2

  • 1College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China.

Archives of Virology
|January 24, 2019
PubMed

Insights

A novel mycovirus, Fusarium incarnatum alternavirus 1 (FiAV1), was discovered in peony root rot pathogen Fusarium incarnatum. This finding expands the known diversity of the proposed Alternaviridae family.

Area of Science:

  • Mycology
  • Virology
  • Plant Pathology

Background:

  • Fusarium incarnatum causes peony root rot.
  • Mycoviruses, viruses that infect fungi, can influence fungal biology and pathogenicity.
  • The proposed family Alternaviridae comprises dsRNA viruses with segmented genomes.

Purpose of the Study:

  • To characterize a novel mycovirus identified in Fusarium incarnatum.
  • To determine the genomic composition and phylogenetic relationships of the new virus.

Main Methods:

  • Isolation and characterization of a double-stranded RNA (dsRNA) mycovirus from Fusarium incarnatum.
  • Whole-genome sequencing of the three dsRNA segments.
  • Bioinformatic analysis, including multiple sequence alignment and phylogenetic analysis of RNA-dependent RNA polymerase (RdRp) amino acid sequences.

Main Results:

  • A novel dsRNA mycovirus, Fusarium incarnatum alternavirus 1 (FiAV1), was identified in F. incarnatum.
  • FiAV1 possesses a three-segment dsRNA genome (dsRNA1, dsRNA2, dsRNA3) with conserved genomic features of Alternaviridae, including poly(A) tails and a specific 5 ahydro-terminal nonamer sequence.
  • Phylogenetic analysis suggests FiAV1 represents a new strain within a potential new species in the proposed "Fusarium alternavirus 1" genus.

Conclusions:

  • FiAV1 is the first reported mycovirus from the proposed "Alternaviridae" family to infect Fusarium incarnatum.
  • This discovery expands the host range and known diversity of alternaviruses.
  • Further research on FiAV1 may elucidate its impact on F. incarnatum pathogenicity and fungal evolution.

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