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Updated: Jan 30, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Published on: May 28, 2014
Rapid Identification of Bacteria by Membrane-Responsive Aggregation of a Pyrene Derivative
Shuangshuang Long1,2, Lu Miao1, Ruihua Li3
1CAS Key Laboratory of Separation Science for Analytical Chemistry , Dalian Institute of Chemical Physics , Chinese Academy of Sciences , Dalian 116023 , China.
Abstract:
An imidazolium-derived pyrene aggregation was developed to rapidly identify and quantify different bacteria species. When the nonemissive aggregates bound to the anionic bacteria surface, the sensor disassembled to turn on significant fluorescence. At the same time, ratiometric signals between pyrene monomer and excimer emission were controlled by different interactions with various bacteria surfaces. The resulted different fluorescent emission profiles then were obtained as fingerprints for various bacterial species. By converting emission profiles directly into output signals of two channels, fluorescence increase and ratiometric change, a two-dimensional analysis map was generated for bacteria identification. We demonstrated that our sensor rapidly identified 10 species of bacteria and 14 clinical isolated multidrug-resistant bacteria, and we determined their staining properties (Gram-positive or Gram-negative).
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