Related Experiment Video

Updated: Jan 30, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

1.1K

Ailanthone reverses multidrug resistance by inhibiting the P-glycoprotein-mediated efflux in resistant K562/A02 cells

Fang Han1, Guoqiang Liu1, Caifeng Sun1

  • 1Hematology Department, Shengli Oilfield Central Hospital, No. 31 Ji'nan Road, Dongying District, Dongying, Shandong, 257034, China.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|January 24, 2019
PubMed

Insights

Ailanthone (AIL) reverses multidrug resistance (MDR) in cancer by inhibiting P-glycoprotein (P-GLP). This natural compound restores cancer cell sensitivity to chemotherapy drugs like doxorubicin (DOX).

Area of Science:

  • Pharmacology
  • Biochemistry
  • Oncology

Background:

  • Multidrug resistance (MDR) significantly hinders effective cancer chemotherapy.
  • Overexpression of P-glycoprotein (P-GLP), an ATP-binding cassette transporter, is a primary mechanism of MDR.
  • There is a critical need for novel agents to overcome P-GLP-mediated drug resistance.

Purpose of the Study:

  • To investigate the potential of ailanthone (AIL), a natural compound, in reversing P-GLP-induced MDR.
  • To elucidate the mechanisms by which AIL overcomes P-GLP-mediated drug resistance in cancer cells.
  • To evaluate AIL's efficacy in restoring sensitivity to doxorubicin (DOX).

Main Methods:

  • Treatment of K562/A02 cells with ailanthone (AIL) and doxorubicin (DOX).
  • Measurement of intracellular doxorubicin (DOX) accumulation and rhodamine 123 (Rh123) efflux.
  • Assessment of P-glycoprotein (P-GLP) expression, P-GLP ATPase activity, and MDR1 gene expression.
  • Investigation of the involvement of the PI3K/Akt signaling pathway in AIL's mechanism of action.

Main Results:

  • Ailanthone (AIL) effectively reversed P-glycoprotein (P-GLP)-mediated multidrug resistance (MDR).
  • AIL increased intracellular doxorubicin (DOX) accumulation and inhibited Rh123 efflux by suppressing P-GLP.
  • AIL suppressed P-GLP ATPase activity, reduced P-GLP protein and MDR1 gene expression, and inhibited the PI3K/Akt pathway.

Conclusions:

  • Ailanthone (AIL) demonstrates significant potential as a therapeutic agent for reversing P-glycoprotein (P-GLP)-mediated multidrug resistance (MDR) in cancer.
  • AIL restores cancer cell sensitivity to doxorubicin (DOX) by down-regulating P-GLP expression and activity via the PI3K/Akt pathway.
  • AIL represents a promising natural compound for developing novel strategies against drug-resistant cancers.
Keywords:
AilanthoneP-glycoprotein-mediated efflux in resistant K562/A02 cells.multidrug resistance

More Related Videos

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis23:06

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

Published on: August 11, 2008

19.6K
In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging09:29

In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging

Published on: April 28, 2017

9.9K

Related Concept Videos

Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.5K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K
Equivalent Resistance01:16

Equivalent Resistance

In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
977
Resistance and Conductance01:25

Resistance and Conductance

A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
510
Rolling Resistance01:21

Rolling Resistance

When a solid cylinder rolls steadily on a rigid surface, the normal force applied by the surface on the cylinder is perpendicular to the tangent at the contact point. However, since no materials are entirely rigid, the surface's reaction to the cylinder involves a range of normal pressures.
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
659
Vascular Resistance01:20

Vascular Resistance

Vascular resistance is a critical concept in understanding blood flow dynamics in the circulatory system. It refers to the resistance that blood encounters as it flows through the blood vessels. This resistance is a key factor in determining blood pressure and cardiac workload.
The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...
10.8K