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Updated: Jan 30, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

A dynamic DNA nanostructure with switchable and size-selective molecular recognition properties.

Chan-Jin Kim¹, Eun Hye Jeong, Hyukjin Lee

¹Department of Chemistry and Nanoscience, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Republic of Korea. sojungpark@ewha.ac.kr.

|January 24, 2019

Summary

Researchers created dynamic DNA nanostructures with tunable recognition properties. These hybrid micelles offer switchable, size-selective interactions controllable by temperature, impacting cellular uptake and degradation.

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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

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Published on: October 8, 2019

Area of Science:

Nanotechnology
Biomaterials Science
Polymer Chemistry

Background:

Developing dynamic nanostructures with controllable molecular recognition is crucial for advanced applications.
Block copolymers offer versatile platforms for creating complex self-assembled architectures.

Purpose of the Study:

To engineer a dynamic DNA nanostructure with switchable and size-selective molecular recognition capabilities.
To investigate the role of thermo-responsive polymers in controlling nanostructure interactions.

Main Methods:

Simultaneous self-assembly of polystyrene-b-DNA (PS-b-DNA) and PS-b-poly(N-isopropylacrylamide) (PS-b-PNIPAM) into hybrid micelles.
Characterization of micelle structure and evaluation of molecular and nanoscale species interactions.
Assessment of temperature-dependent changes in steric hindrance and biological functions.

Main Results:

Hybrid micelles formed with a PS core and a DNA/PNIPAM corona.
PNIPAM provided tunable steric hindrance, blocking nanoscale species interactions above the lower critical solution temperature (LCST).
Demonstrated thermally controllable enzymatic degradation and cellular uptake of the hybrid nanostructures.

Conclusions:

Binary self-assembly of responsive block copolymers enables the creation of dynamic nanostructures.
These nanostructures exhibit switchable, size-selective recognition properties with potential in biological applications.
Temperature control offers a viable strategy for modulating nanostructure-biomolecule interactions.