Related Experiment Video
Updated: Jan 30, 2026

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
Published on: August 1, 2015
HPLC-UV-MS Analysis: A Source for Severe Oxidation Artifacts
Fritz Schweikart1, Gustaf Hulthe2
1Advanced Drug Delivery, Pharmaceutical Sciences, IMED Biotech Unit , AstraZeneca , Gothenburg 43183 , Sweden.
Abstract:
HPLC coupled to both UV and MS is an established setup for purity assessments in many areas. With evolving technology, instrument sensitivity increases, calling for lower sample concentration, while light flux in a commercial UV detector cell is considerably higher than earlier. This evolution has now reached the point where radicals formed by UV light are abundant enough, compared to the analyte levels, to generate unwanted artifact signals in the MS spectrum. In this work we show several examples from pharmaceutical development where UV degradation in the UV detector leads to severely misleading mass spectra in typical day to day samples.
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