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Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling
Luisa Galla1,2, Paola Pizzo1,2, Elisa Greotti3,4
1Department of Biomedical Sciences, University of Padua, Padua, Italy.
Abstract:
Calcium ion (Ca2+) is a ubiquitous intracellular messenger able to generate versatile intracellular signals that modulate a large variety of functions in virtually every cell type. Chemical and genetic biosensors, targeted to different subcellular compartments, have been developed and continuously improved to monitor Ca2+ dynamics in living cells. Here we describe the usage of Förster resonance energy transfer (FRET)-based Cameleon probes to investigate Ca2+ influx across the plasma membrane (PM) or Ca2+ release from the main intracellular Ca2+ store, the endoplasmic reticulum (ER).
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