Imaging Intracellular Ca²⁺ in Cardiomyocytes with Genetically Encoded Fluorescent Probes

Calcium (Ca²⁺) is a key player in cardiomyocyte homeostasis, and its roles span from excitation-contraction coupling to metabolic and structural signaling. Alterations in the function or expression of Ca²⁺-handling proteins are common findings in failing cardiomyocytes, which have been linked to impaired contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca²⁺ handling in cardiomyocytes represents a central method in experimental molecular cardiology.