Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Circular replicating baculovirus genome].

I P Kok, I N Skuratovskaia

    Tsitologiia I Genetika
    |September 1, 1988
    PubMed
    Summary
    This summary is machine-generated.

    Free Malacosoma neustria nuclear polyhedrosis virus preparations reveal insights into Baculovirus replication. The study identifies intermediate DNA molecules and their role in forming virus-like particles.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Development of the molecular genetics of baculoviruses in Ukraine].

    TSitologiia i genetika·1986
    Same author

    [Physico-chemical characteristics of the DNA of the nuclear polyhedrosis virus of the moth Porthetria dispar L].

    Ukrainskii biokhimicheskii zhurnal (1978)·1984
    Same author

    [A non-damaging procedure for DNA isolation from insect cell nuclei].

    Ukrainskii biokhimicheskii zhurnal (1978)·1982
    Same author

    Macromolecular structure of nuclear polyhedrosis virus genome.

    Archives of virology·1979
    Same author

    [DNA synthesis on the heterogeneous nuclear RNA template catalysed by DNA polymerase of avian myeloblastosis virus].

    Molekuliarnaia biologiia·1975
    Same author

    [Sedimentation properties of virus-specific RNA in nuclear polyhedrosis of the bee moth].

    Mikrobiolohichnyi zhurnal·1975

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Context:

    • Baculoviruses are DNA viruses widely used as expression vectors.
    • Understanding their replication cycle is crucial for optimizing their use.
    • Malacosoma neustria nuclear polyhedrosis virus (MnNPV) serves as a model system.

    Purpose:

    • To characterize the morphology and DNA forms of MnNPV.
    • To investigate the replication intermediates of viral DNA.
    • To elucidate the formation of virus-like particles.

    Summary:

    • Free MnNPV preparations contain nucleocapsids and virus-like particles.
    • Viral DNA is circular and covalently closed, with preparations showing single-stranded breaks, catenanes, and circular dimers.
    • Replicating DNA, including theta-forms, catenanes, and circular dimers, were identified as intermediate molecules in heavy sucrose gradient fractions.

    Related Experiment Videos

    Impact:

    • Provides a detailed understanding of Baculovirus DNA replication intermediates.
    • Suggests a mechanism for the formation of virus-like particles involving protein complexes and inhibited monomerization.
    • Contributes to the fundamental knowledge of viral DNA assembly and morphogenesis.