The kinetics of cell division in a synchronizing fermentor

The kinetics of cell proliferation of Candida tropicalis synchronized by periodical glucose starvation was examined in a continuous synchronizing fermentor. The length of the deterministic (B) and stochastic (A) phase of the cell cycle was determined by cell density data analysis using the Transition Probability Model. The size distribution of the population shows that the absence of nutrient stops the cells at the beginning of the cell cycle but the succeeding cell divisions are not perfectly synchronized. The reason of this comes from the difference between the generation times of the cells which is the consequence of the different long "A" phases in their cycle.