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Insulin-like growth factor 1 modulates bioengineered tooth morphogenesis.

Toshihito Oyanagi1, Nobuo Takeshita1, Mamiko Hara1

  • 1Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, 980-0875, Japan.

Scientific Reports
|January 25, 2019
PubMed
Summary
This summary is machine-generated.

Insulin-like growth factor 1 (IGF1) enhances the size and shape of bioengineered teeth. This regenerative therapy approach promotes normal tooth development by increasing enamel knots and cell proliferation.

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Area of Science:

  • Biotechnology
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Regenerative therapy for missing teeth is crucial.
  • Current bioengineered teeth are smaller and abnormally shaped compared to natural teeth.

Purpose of the Study:

  • To investigate if insulin-like growth factor 1 (IGF1) can regulate the size and shape of bioengineered teeth.
  • To understand the mechanisms behind IGF1's regulatory effects.

Main Methods:

  • Organ germ method using mouse tooth germ cells.
  • Treatment with IGF1.
  • Development of bioengineered tooth germs in subrenal capsules and jawbones.

Main Results:

  • IGF1 treatment significantly increased bioengineered tooth germ size while maintaining normal histology.
  • IGF1-enhanced teeth exhibited larger sizes and more cusps.
  • IGF1 increased fibroblast growth factor 4 (Fgf4)-expressing enamel knots and promoted dental cell proliferation and differentiation.

Conclusions:

  • IGF1 is a key regulator for increasing the size and cusp number of bioengineered teeth.
  • IGF1 promotes tooth regeneration by inducing enamel knot formation and enhancing dental cell proliferation and differentiation.