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Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells

Mingde Ding1, Hui Zhang1, Lei Liu2

  • 1Department of Gynaecology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China.

Oncology Letters
|January 25, 2019
PubMed

Insights

Nitric oxide synthase 1 (NOS1) and ABCG2 are elevated in cervical cancer, promoting tumor growth. NOS1 regulates ABCG2, impacting cancer cell proliferation and apoptosis, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Cervical cancer is a significant global health concern.
  • The roles of nitric oxide synthase 1 (NOS1) and adenosine triphosphate-binding cassette sub-family G member 2 (ABCG2) in cervical cancer remain incompletely understood.
  • Investigating the interplay between NOS1 and ABCG2 may reveal novel therapeutic strategies.

Purpose of the Study:

  • To investigate the expression levels of NOS1 and ABCG2 in cervical cancer tissues.
  • To analyze the correlation between NOS1 and ABCG2 expression.
  • To elucidate the regulatory relationship between NOS1 and ABCG2 and their impact on cervical cancer cell proliferation and apoptosis.

Main Methods:

  • Quantitative analysis of NOS1 and ABCG2 mRNA expression using reverse transcription-polymerase chain reaction (RT-PCR).
  • Protein expression analysis via Western blotting.
  • In vitro studies using cervical cancer cell lines (HeLa, C-33A) with lentiviral transfection for gene interference.

Main Results:

  • Significantly elevated mRNA levels of NOS1 and ABCG2 were observed in cervical cancer tissues compared to normal controls (P<0.05).
  • A positive correlation was found between NOS1 and ABCG2 mRNA expression (r=1.246, P=0.014).
  • NOS1 positively regulates ABCG2 protein expression; NOS1 interference decreased proliferation and increased apoptosis in cervical cancer cells, while ABCG2 interference did not affect NOS1 levels.

Conclusions:

  • Both NOS1 and ABCG2 are upregulated in cervical cancer and function as oncogenes promoting tumor growth.
  • NOS1 acts as an upstream regulator of ABCG2, influencing cervical cancer cell proliferation and apoptosis.
  • Targeting NOS1 and ABCG2 may offer a promising therapeutic avenue for cervical cancer treatment.
Keywords:
ABCG2NOS1apoptosiscervical cancerproliferation

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