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Analyses on K-ras mutations and fascin expression in patients with cardia cancer

Li Wei1, Haiyan Chang2, Song Huo3

  • 1Department of Pathology, Jining First People's Hospital, Jining, Shandong 272111, P.R. China.

Oncology Letters
|January 25, 2019
PubMed

Insights

Cardia cancer patients with K-ras gene mutations, particularly at codon 12, show significantly higher fascin protein expression. Alcohol abuse is identified as a risk factor for these K-ras mutations.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cardia cancer is a significant health concern globally.
  • K-ras gene mutations are implicated in various cancers.
  • Fascin is a protein involved in cell motility and cancer progression.

Purpose of the Study:

  • To investigate K-ras gene mutations in cardia cancer patients.
  • To analyze the effect of K-ras mutations on fascin expression.
  • To identify potential risk factors for K-ras mutations in cardia cancer.

Main Methods:

  • Genomic DNA extraction from cardia cancer tissues.
  • Pyrosequencing and quantitative polymerase chain reaction (qPCR) for K-ras mutation detection.
  • Immunohistochemistry and qPCR for fascin expression analysis.

Main Results:

  • K-ras mutations were found in 23.3% of cardia cancer patients, predominantly in exon 12.
  • Alcohol abuse was identified as a significant risk factor for K-ras mutations.
  • Fascin expression was significantly higher (2.3-fold) in patients with K-ras mutations compared to those without.

Conclusions:

  • Codon 12 mutations in the K-ras gene are common in cardia cancer.
  • Fascin is highly expressed in cardia cancer patients with K-ras mutations.
  • Fascin expression is positively correlated with K-ras gene mutations in cardia cancer.
Keywords:
K-rat sarcomacardia cancerfascinmutation

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