Related Experiment Video
Updated: Jan 30, 2026

MALDI Sample Preparation: the Ultra Thin Layer Method
Published on: April 29, 2007
Combining MALDI-2 and transmission geometry laser optics to achieve high sensitivity for ultra-high spatial
Eric C Spivey1,2, Josiah C McMillen1,3, Daniel J Ryan1,3
1Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.
Abstract:
A transmission geometry optical configuration allows for smaller laser spot size to facilitate high-resolution matrix-assisted laser/desorption ionization (MALDI) mass spectrometry. This increase in spatial resolution (ie, smaller laser spot size) is often associated with a decrease in analyte signal. MALDI-2 is a post-ionization technique, which irradiates ions and neutrals generated in the initial MALDI plume with a second orthogonal laser pulse, and has been shown to improve sensitivity. Herein, we have modified a commercial Orbitrap mass spectrometer to incorporate a transmission geometry MALDI source with MALDI-2 capabilities to improve sensitivity at higher spatial resolutions.
Related Concept Videos
Coordination Number and Geometry
Matrix-Assisted Laser Desorption Ionization (MALDI)
Predicting Molecular Geometry
Geometry of Hyperbolas
Design of Transmission Shafts - Stress Analysis
Molecular Geometry and Dipole Moments

