Localization of inhibin in human testes by immunoperoxidase technique

Immunohistological studies on the localization of inhibin in human testes were carried out to investigate the cell types involved in inhibin synthesis/storage. The paraffin embedded testicular tissues were stained by an indirect immunoperoxidase technique. Specific antiserum to human testicular inhibin generated against the homogenous preparation of human testicular inhibin at our laboratory was used as the first antibody. Inhibin was found to be localized in Sertoli cells, spermatogonia, and primary spermatocytes. A weak positive reaction was also observed in spermatids. Interestingly, Leydig cells showed positive reaction indicating presence of inhibin in these cells too.