Pseudaminobacter arsenicus sp. nov., an arsenic-resistant bacterium isolated from arsenic-rich aquifers

An arsenic-resistant strain, CB3^T, was isolated from arsenic-rich aquifers at the Jianghan Plain in Hubei, China. Phylogenetic and biochemical analysis suggested that it should represent a new species of the genus Pseudaminobacter in the family Phyllobacteriaceae. The 16S rRNA gene of CB3^T shared the highest sequence similarities to those of the type strains Pseudaminobacter defluvii THI 051^T (97.8 % identity) and Pseudaminobacter salicylatoxidans BN12^T (97.4 %). The DNA-DNA relatedness values of CB3^T with respect to strains belonging to the genus Pseudaminobacter were less than 70 %. The fatty acid profile of CB3^T consisted of C16 : 0, cyclo-C19 : 0ω8c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as major components. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 61.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CB3^T was distinct from previously described Pseudaminobacter species. Therefore, we propose that strain CB3^T represents a novel species of the genus Pseudaminobacter, Pseudaminobacterarsenicus sp. nov., strain CB3^T (=CCTCC AB2016116^T=KCTC 52625^T) is designated as the type strain.