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Related Experiment Video

Updated: Jan 30, 2026

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
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Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions.

Yun Ge1, Long Chen1, Shibo Liu1

  • 1Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering , Peking University , Beijing , China.

Journal of the American Chemical Society
|January 25, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed enzyme-mediated proximity cell labeling (EXCELL) to detect cell communications. This method uses a novel enzyme to label interacting cells, offering new insights into cell-cell interactions.

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Area of Science:

  • Cell biology
  • Biochemistry
  • Molecular biology

Background:

  • Cell-cell interactions are crucial for life but poorly understood.
  • Existing methods have limitations in studying these interactions in real-time.

Purpose of the Study:

  • To develop a novel strategy for detecting and recording cell-cell interactions.
  • To establish a general method applicable under living conditions.

Main Methods:

  • Developed an enzyme-mediated proximity cell labeling (EXCELL) strategy.
  • Utilized an evolved Staphylococcus aureus transpeptidase sortase A variant (mgSrtA).
  • mgSrtA promiscuously labels N-terminal monoglycine-containing cell surface proteins.

Main Results:

  • EXCELL enables proximity-dependent labeling of interacting cells.
  • The method allows for the detection of cell-cell communications in living systems.
  • Demonstrated a general approach for studying cell interactions.

Conclusions:

  • EXCELL is a versatile tool for investigating cell-cell interactions.
  • This strategy advances the study of cellular communication dynamics.
  • Provides a foundation for future research in cell-cell communication.