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Updated: Jan 30, 2026

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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miR-424-5p Regulates Hepatoma Cell Proliferation and Apoptosis

Lianshu Piao1, Fei Wang1, Yanyan Wang1

  • 1Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.

Cancer Biotherapy & Radiopharmaceuticals
|January 25, 2019
PubMed
Abstract

Insights

Reduced miR-424-5p expression is linked to increased Yes-associated protein 1 (YAP1) in liver cancer. Restoring miR-424-5p inhibits YAP1, reducing hepatoma cell proliferation and promoting apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Yes-associated protein 1 (YAP1) is a key oncogene in liver cancer pathogenesis and progression.
  • MicroRNA (miR)-424-5p expression is significantly reduced in liver cancer tissues, suggesting an anticancer role.

Purpose of the Study:

  • To investigate the regulatory relationship between miR-424-5p and YAP1 in liver cancer.
  • To determine the effect of miR-424-5p on hepatoma cell proliferation and apoptosis.

Main Methods:

  • Analysis of miR-424-5p and YAP1 expression in patient tissues and cell lines.
  • Dual-luciferase reporter gene assay to confirm direct targeting of YAP1 by miR-424-5p.
  • In vitro experiments using miR-424-5p mimics to assess effects on cell proliferation and apoptosis.

Main Results:

  • Liver cancer tissues and cells exhibit decreased miR-424-5p and increased YAP1 expression, correlating with clinical stage and malignancy.
  • A negative correlation was observed between miR-424-5p and YAP1 levels.
  • miR-424-5p mimic transfection suppressed YAP1 expression, reduced cell proliferation, and induced apoptosis in hepatoma cells.

Conclusions:

  • Downregulation of miR-424-5p and upregulation of YAP1 are characteristic of liver cancer.
  • Increasing miR-424-5p expression can suppress YAP1, inhibit hepatoma cell proliferation, and induce apoptosis, highlighting its therapeutic potential.
Keywords:
YAP1apoptosisliver cancermiR-424-5pproliferation

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