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Chemical synthesis of a gene for human stefin A and its expression in E. coli
M Strauss1, J Stollwerk, B Lenarcic
1Institut für Biochemie, Technische Hochschule Darmstadt.
Biological Chemistry Hoppe-Seyler
|September 1, 1988
Abstract:
A DNA containing the coding sequence for the human cysteine proteinase inhibitor stefin A was obtained by enzymic ligation of chemically synthesized deoxyoligonucleotides, using the Khorana ligation method. The 306-bp synthetic gene carries signals for the initiation and termination of its translation. The gene was expressed in E. coli using a cytoplasmic expression vector and stefin A was secreted under the control of the E. coli alkaline phosphatase signal sequence, respectively. The secreted hybrid protein was shown to exhibit biological properties similar to the native protein isolated from human plasma.