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Updated: Jan 30, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Published on: November 29, 2024
HR-MAS NMR Based Quantitative Metabolomics in Breast Cancer
Mikheil Gogiashvili1, Jessica Nowacki2, Roland Hergenröder3
1Leibniz Institut für Analytische Wissenschaften, ISAS, e.V., 44139 Dortmund, Germany. mikheil.gogiashvili@isas.de.
Abstract:
High resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy is increasingly used for profiling of breast cancer tissue, delivering quantitative information for approximately 40 metabolites. One unique advantage of the method is that it can be used to analyse intact tissue, thereby requiring only minimal sample preparation. Importantly, since the method is non-destructive, it allows further investigations of the same specimen using for instance transcriptomics. Here, we discuss technical aspects critical for a successful analysis - including sample handling, measurement conditions, pulse sequences for one- and two dimensional analysis, and quantification methods - and summarize available studies, with a focus on significant associations of metabolite levels with clinically relevant parameters.
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