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Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
Published on: June 10, 2020
CENP-A Ubiquitylation Contributes to Maintaining the Chromosomal Location of the Centromere
Yohei Niikura1,2, Risa Kitagawa3, Katsumi Kitagawa4
1Greehey Children's Cancer Research Institute, Department of Molecular Medicine, UT Health Science Center San Antonio School of Medicine, 8403 Floyd Curl Drive, San Antonio, TX 78229-3000, USA. niikura@nicemice.cn.
Abstract:
The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)-RING-box protein 1 (RBX1)-COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.
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