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Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal

Zhendong Liu1, Dongsheng Yu2,3, Liang Li4

  • 1Food Science College, Tibet Agriculture & Animal Husbandry University, Linzhi, 860000, China. liu304418091@126.com.

Molecules (Basel, Switzerland)
|January 26, 2019
PubMed

Polysaccharides from the immunomodulatory medicinal mushroom Inonotus obliquus (IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolated yield was identified, using the following protocol: a solid-liquid ratio of 1 g to 12 mL; mass fraction of (NH₄)₂SO₄ 20% (w/v); 11 mL t-butanol; pH 8.0; temperature 30 °C; and extraction time 30 min. The purified IOPS was shown to be a proteoglycan of 40 kDa molecular weight comprising of d-galactose, d-glucose, d-xylose, and d-mannose in a molar ratio of 2.0:3.5:1.0:1.5. The purified IOPS displayed strong free-radical scavenging abilities, antioxidant activities, and immunological activity in vitro. IOPS' Trolox antioxidant equivalent capacity and ferric-reducing ability of plasma were 251.2 μmol Trolox/g sample and 1040.5 μmol Fe2+/g sample, respectively, with the activity of its immunomodulatory behavior shown to be gradient dependent.

Keywords:
Inonotus obliquusantioxidant activityfree-radical scavenging abilitiesimmunological activitythree-phase partitioning

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