Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Replisome03:01

The Replisome

38.3K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
38.3K
The Replisome03:01

The Replisome

10.0K
10.0K
The Uncertainty Principle04:08

The Uncertainty Principle

31.8K
Werner Heisenberg considered the limits of how accurately one can measure properties of an electron or other microscopic particles. He determined that there is a fundamental limit to how accurately one can measure both a particle’s position and its momentum simultaneously. The more accurate the measurement of the momentum of a particle is known, the less accurate the position at that time is known and vice versa. This is what is now called the Heisenberg uncertainty principle. He...
31.8K
Hardy-Weinberg Principle01:49

Hardy-Weinberg Principle

76.3K
Diploid organisms have two alleles of each gene, one from each parent, in their somatic cells. Therefore, each individual contributes two alleles to the gene pool of the population. The gene pool of a population is the sum of every allele of all genes within that population and has some degree of variation. Genetic variation is typically expressed as a relative frequency, which is the percentage of the total population that has a given allele, genotype or phenotype.
76.3K
The Pauli Exclusion Principle03:06

The Pauli Exclusion Principle

59.3K
The arrangement of electrons in the orbitals of an atom is called its electron configuration. We describe an electron configuration with a symbol that contains three pieces of information:
59.3K
The Aufbau Principle and Hund's Rule03:02

The Aufbau Principle and Hund's Rule

72.7K
To determine the electron configuration for any particular atom, we can build the structures in the order of atomic numbers. Beginning with hydrogen, and continuing across the periods of the periodic table, we add one proton at a time to the nucleus and one electron to the proper subshell until we have described the electron configurations of all the elements. This procedure is called the aufbau principle, from the German word aufbau (“to build up”). Each added electron occupies the...
72.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using <i>spIsoNet</i> to address the preferred-orientation problem in cryoEM reconstructions.

bioRxiv : the preprint server for biology·2026
Same author

Structure and potential role of T6SS effector PdpC in Francisella tularensis intracellular lifestyle.

Communications biology·2026
Same author

TASP1-mediated cleavage of REV3L enhances the activity of DNA polymerase ζ in mammalian cells.

Nature communications·2026
Same author

Structural Activation of DNA Unwinding by MCM8/9/HROB.

bioRxiv : the preprint server for biology·2026
Same author

Cryo-EM Structures Reveal Upstream DNA Interactions within the Mitochondrial Transcription Initiation Complex.

bioRxiv : the preprint server for biology·2026
Same author

The tumour suppressor RBM5 activates the helicase DHX15 to regulate splicing.

Research square·2026

Related Experiment Video

Updated: Jan 30, 2026

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

1.7K

Structures and operating principles of the replisome.

Yang Gao1, Yanxiang Cui2, Tara Fox3,4

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Science (New York, N.Y.)
|January 26, 2019
PubMed
Summary

Atomic-level visualization of the bacteriophage T7 replisome reveals its mechanism for DNA replication. Structures show how helicase, polymerase, and primase work together to synthesize leading and lagging DNA strands.

More Related Videos

Principles of Rodent Surgery for the New Surgeon
10:29

Principles of Rodent Surgery for the New Surgeon

Published on: January 6, 2011

117.2K
Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

91.9K

Related Experiment Videos

Last Updated: Jan 30, 2026

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

1.7K
Principles of Rodent Surgery for the New Surgeon
10:29

Principles of Rodent Surgery for the New Surgeon

Published on: January 6, 2011

117.2K
Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

91.9K

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The DNA replisome is essential for DNA replication, coordinating leading and lagging strand synthesis.
  • Atomic-level visualization of the complete replisome has been challenging.

Purpose of the Study:

  • To visualize the bacteriophage T7 replisome at atomic resolution.
  • To elucidate the molecular mechanisms of coupled DNA strand synthesis.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) at 3.2-angstrom resolution.
  • Structural determination of key replisome components and complexes.

Main Results:

  • Detailed structures of helicase translocation and helicase-polymerase-primase complexes.
  • Revealed sequential, hand-over-hand translocation of the hexameric helicase.
  • Identified two lagging-strand polymerases associated with primase for Okazaki fragment synthesis.
  • Showcased a β hairpin from the leading-strand polymerase separating parental DNA strands.

Conclusions:

  • The T7 replisome operates through a coordinated mechanism involving sequential helicase translocation and coupled DNA synthesis.
  • Structural insights provide a molecular basis for understanding DNA replication fork dynamics.