Ag(I) and Au(III) Mercaptobenzothiazole complexes induced apoptotic cell death
Jositta Sherine1, Arun Upadhyay2, Amit Mishra2
1Department of Physics and Nanotechnology, SRM Institute of Science and Technology, Kattankulathur, 603203, India.
Silver(I) and Gold(III) 2-mercaptobenzothiazole (MBT) complexes were synthesized and studied. Ag(I)MBT demonstrated higher induced apoptosis in A549 cells compared to Au(III)MBT, suggesting potential as anti-proliferating agents.
Area of Science:
- Coordination Chemistry
- Materials Science
- Biomedical Research
Background:
- 2-Mercaptobenzothiazole (MBT) is a versatile ligand with potential applications.
- Metal complexes, particularly of Ag(I) and Au(III), are explored for their biological activities.
- Understanding the cytotoxic effects of novel metal-MBT complexes is crucial for therapeutic development.
Purpose of the Study:
- To synthesize and characterize novel Ag(I) and Au(III) complexes with 2-mercaptobenzothiazole (MBT).
- To investigate the structural, optical, spectroscopic, and electrochemical properties of these metal complexes.
- To evaluate the potential of Ag(I)MBT and Au(III)MBT complexes in inducing apoptosis in cancer cells.
Main Methods:
- Wet chemical synthesis of Ag(I)MBT and Au(III)MBT complexes.
- Comprehensive characterization using techniques such as ICP-MS, 1HNMR, optical spectroscopy, and electrochemical analysis.
- In vitro apoptosis induction assessment using TUNEL assay on A549 cell lines.
Main Results:
- Successful synthesis and structural elucidation of Ag(I)MBT and Au(III)MBT complexes.
- Ag(I)MBT and Au(III)MBT complexes exhibited distinct spectroscopic and electrochemical profiles.
- TUNEL assay revealed significant apoptosis induction by both complexes, with Ag(I)MBT showing higher efficacy (60% cell viability) compared to Au(III)MBT (80% cell viability).
Conclusions:
- The synthesized Ag(I)MBT and Au(III)MBT complexes possess unique properties.
- Ag(I)MBT exhibits a greater capacity to induce apoptosis in A549 cells than Au(III)MBT.
- These MBT metal complexes represent promising candidates for development as anti-proliferating agents.
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