Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Bonds02:43

Peptide Bonds

82.8K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
82.8K
Gene Therapy00:59

Gene Therapy

27.6K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.6K
Vectors01:30

Vectors

478
Vectors are mathematical entities characterized by both magnitude and direction. Unlike scalars, which are defined solely by magnitude, vectors represent quantities like displacement, velocity, and force, where direction is essential. Vectors are graphically represented as directed line segments, extending from an initial point to a terminal point, denoted with bold letters or arrows placed above the symbol. Two vectors are deemed equal if they share identical magnitudes and directions,...
478
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.6K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.6K
Impact Loading01:19

Impact Loading

691
Impact loading occurs when a moving object collides with a stationary structure, such as a rod with a uniform cross-sectional area fixed at one end. Under these conditions, the rod absorbs the kinetic energy from the striking object, leading to deformation and subsequent stress development. As the rod returns to its original position and reaches maximum stress, the absorbed energy, initially manifested as kinetic energy, transforms entirely into strain energy.
In cases of elastic deformation,...
691
Distributed Loads01:19

Distributed Loads

969
Distributed loads are a common type of load that engineers and scientists encounter in various practical situations. Distributed loads often refer to a type of load spread over a surface or a structure and can be modeled as continuous force per unit area.
For example, consider a bookshelf filled with books stacked vertically adjacent to each other. The weight of the books is evenly distributed over the length of the shelf. As a result, the pressure at different locations on the surface of the...
969

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Polycation-Carbon Nanohybrids with Superior Rough Hollow Morphology for the NIR-II Responsive Multimodal Therapy".

ACS applied materials & interfaces·2026
Same author

Minimizing Implant Rejections through Low-Inflammatory and Immune-Regulatory Biointerfaces.

ACS applied materials & interfaces·2026
Same author

Silicon(IV) Phthalocyanine-Functionalized Hyaluronic Acid for Photodynamic Eradication of Methicillin-Resistant <i>Staphylococcus aureus</i> Infections.

ACS applied materials & interfaces·2026
Same author

Correction: MicroRNA-mediated silence of onco-lncRNA MALAT1 in different ESCC cells <i>via</i> ligand-functionalized hydroxyl-rich nanovectors.

Nanoscale·2026
Same author

High-biocompatibility chitosan-based vectors for high-dose gene therapy in tumor suppression and wound healing.

Biomaterials·2026
Same author

Forced hemostasis <i>via</i> enhanced erythrocyte adhesion: A procyanidin-based composite sponge for managing hemorrhage in coagulopathic conditions.

Materials today. Bio·2026

Related Experiment Video

Updated: Jan 30, 2026

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
08:42

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Published on: January 15, 2018

11.7K

Peptide-grafted dextran vectors for efficient and high-loading gene delivery.

Yang Hu1, Huifeng Wang, Haiqing Song

  • 1State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.

Biomaterials Science
|January 26, 2019
PubMed
Summary

Histidine-rich peptide-grafted dextran vectors show high gene transfection efficiency. This histidine-rich material enhances DNA binding and endosomal escape, outperforming PEI at low N/P ratios.

More Related Videos

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
12:30

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Published on: October 6, 2017

7.2K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.4K

Related Experiment Videos

Last Updated: Jan 30, 2026

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
08:42

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Published on: January 15, 2018

11.7K
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
12:30

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Published on: October 6, 2017

7.2K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.4K

Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Polymer Chemistry

Background:

  • Peptide-based vectors offer unique advantages for gene delivery.
  • A deeper molecular understanding of amino acid roles in gene delivery is needed.
  • Dextran-grafted peptides present a promising avenue for gene delivery systems.

Purpose of the Study:

  • To investigate the role of histidine in peptide-grafted dextran gene delivery vectors.
  • To synthesize and characterize a series of histidine-containing peptide-grafted dextran (D-RxHy) vectors.
  • To evaluate the gene transfection efficiency, DNA binding, and cell uptake of these novel vectors.

Main Methods:

  • Synthesis of D-RxHy vectors by grafting RxHyC peptides onto dextran.
  • Gel electrophoresis to assess DNA-binding capabilities.
  • In vitro transfection assays using luciferase reporter gene.
  • Cytotoxicity and cell uptake studies.
  • Chloroquine-treated assays to investigate endosomal escape mechanisms.

Main Results:

  • Histidine residues were confirmed to be crucial for DNA binding.
  • All D-RxHy vectors exhibited low cytotoxicity.
  • D-R3H7 demonstrated superior gene transfection efficiency, outperforming branched PEI (25k) by 6-8 fold at a low N/P ratio of 2.
  • D-R3H7 and D-R5H5 showed enhanced cell uptake.
  • High buffering capacity of histidine was identified as key to efficient endosomal escape.

Conclusions:

  • Histidine is critical for both DNA condensation and endosomal escape in gene delivery.
  • The D-RxHy platform offers tunable properties for effective gene delivery.
  • This study provides insights into the molecular mechanisms of polymer-based transfection and highlights potential therapeutic applications.