Related Experiment Video
Updated: Jan 30, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor
Takashi Kanamori1, Yoshiaki Masaki, Yuki Oda
1Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. kseio@bio.titech.ac.jp.
Abstract:
Fluorescence turn-on sensors for adenosine were developed using DNA triplexes modified with a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl)deoxyuridine (dUMBF) and abasic sites. Binding of adenosine to the abasic site next to the dUMBF changed the microenvironment and conformation (from the twisted to planar state) of dUMBF and enhanced the fluorescence. Adenosine could be selectively detected over other nucleosides and adenosine phosphates. The binding of adenosine was confirmed by UV-thermal melting experiments. Further, the conformational changes of dUMBF from the twisted to coplanar state upon binding of adenosine was supported by MD simulations.
More Related Videos
08:44Fluorescent In Situ Hybridization and 5-Ethynyl-2'-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum
Published on: August 3, 2022
15:10From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014
Related Concept Videos
DNA Base Pairing
DNA Base Pairing
ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH3
Chemotherapy-Induced Nausea and Vomiting: 5-HT3 Receptor Antagonists
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Atomic Fluorescence Spectroscopy