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Application of proteomics in studying bacterial persistence

Jordy Evan Sulaiman1, Henry Lam1

  • 1a Department of Chemical and Biological Engineering , The Hong Kong University of Science & Technology , Kowloon , Hong Kong.

Expert Review of Proteomics
|January 26, 2019
PubMed
Abstract

Insights

Bacterial persisters are resilient cells surviving antibiotics. Proteomics offers a powerful approach to study these cells, despite current technical challenges, with recent advances enabling new research opportunities.

Area of Science:

  • Microbiology
  • Proteomics
  • Bacterial Persistence

Background:

  • Bacterial persisters are a small subpopulation of cells that survive antibiotic treatment by entering a dormant state.
  • Persistence poses a significant clinical challenge due to treatment failures and recurrent infections.
  • Understanding the molecular mechanisms underlying persistence is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To review the current understanding of bacterial persistence.
  • To explore the application of proteomics in studying persister cells.
  • To discuss the challenges and future perspectives of using proteomics to investigate bacterial persistence.

Main Methods:

  • Literature review of existing proteomics studies on bacterial persisters.
  • Analysis of technical challenges and recent advancements in persister cell isolation and enrichment.
  • Discussion of the potential of proteomics to elucidate persister cell biology.

Main Results:

  • Limited proteomics studies have been conducted on bacterial persisters to date.
  • Technical challenges in isolating and analyzing persister cells have hindered proteomic investigations.
  • Recent advancements in persister enrichment methods present new opportunities for proteomic research.

Conclusions:

  • Proteomics is a promising tool for unraveling the complexities of bacterial persistence.
  • Overcoming current technical hurdles is essential for advancing our understanding of persister cells.
  • The opportune timing, with new isolation techniques, makes proteomics a key strategy to address bacterial persistence.
Keywords:
Proteomicsantibioticpersistence

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