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Multi-vendor standardized sequence for edited magnetic resonance spectroscopy.

Muhammad G Saleh1, Daniel Rimbault2, Mark Mikkelsen1

  • 1Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA.

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Summary

A new universal MEGA-PRESS sequence with HERMES functionality standardizes spectral editing across MRI vendors. This improves metabolite measurements like GABA, GSH, and Lac for brain research.

Keywords:
Edited MRSHERMESMEGA-PRESSMagnetic resonance spectroscopyStandardization

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Area of Science:

  • Neuroimaging
  • Magnetic Resonance Spectroscopy (MRS)
  • Metabolomics

Background:

  • Spectral editing techniques like MEGA-PRESS are crucial for measuring low-concentration brain metabolites (GABA, GSH, Lac).
  • Existing MEGA-PRESS implementations vary across MRI vendors, leading to inconsistent results.
  • Standardization is needed for reliable inter-vendor metabolite quantification in neuroscience research.

Purpose of the Study:

  • To develop and validate a universal MEGA-PRESS sequence with HERMES functionality for major MRI vendors (Philips, Siemens, GE, Canon).
  • To standardize RF pulse parameters for consistent metabolite editing across platforms.
  • To assess the inter-vendor reproducibility of edited spectra for GABA, GSH, and Lac.

Main Methods:

  • Generation of new RF pulses for a universal MEGA-PRESS sequence with HERMES capability.
  • Phantom and in vivo experiments on Philips, Siemens, GE, and Canon 3T MRI scanners.
  • Comparison of vendor-native MEGA-PRESS sequences with the universal sequence for GABA, Lac, and GSH editing at various echo times (TE).

Main Results:

  • The universal sequence demonstrated improved inter-vendor similarity for GABA- and Lac-edited spectra.
  • The universal HERMES sequence provided crosstalk-free GABA and GSH edited spectra with strong vendor agreement.
  • In vivo metabolite ratios (GABA/Cr, Lac/Cr, GSH/Cr) showed low variation across scanners using the universal sequence.

Conclusions:

  • The developed universal MEGA-PRESS sequence with HERMES functionality is successfully implemented across four major MRI vendors.
  • This standardization enables reliable spectral editing of key brain metabolites (GABA, GSH, Lac) across different platforms.
  • The universal sequence facilitates more consistent and comparable quantitative MRS studies in neuroscience and clinical applications.