Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.6K
3.6K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.5K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.5K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.6K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.6K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic calcium-potassium balancing by KCNMA1 preserves the epithelial/mesenchymal hybrid state and modulates therapy response in ovarian cancer.

Cell death discovery·2026
Same author

Author Correction: Maintenance of R-loop structures by phosphorylated hTERT preserves genome integrity.

Nature cell biology·2026
Same author

Condensin II collaborates with cohesin to establish and maintain interphase chromosome territories.

The Journal of cell biology·2026
Same author

Caspase-8 is a novel modulator of Homologous Recombination Repair in response to ionizing radiations in glioblastoma.

Cancer letters·2025
Same author

The HP1 hinge region: more than just a linker for heterochromatin.

Journal of biochemistry·2025
Same author

Editorial overview: Fresh views on nuclear structure, function, and dynamics.

Current opinion in cell biology·2025

Related Experiment Video

Updated: Jan 30, 2026

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.8K

Non-coding RNAs and chromatin domains.

Tatsuro Yamamoto1, Noriko Saitoh2

  • 1Division of Cancer Biology, The Cancer Institute of JFCR, Tokyo 135-8550, Japan; Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan.

Current Opinion in Cell Biology
|January 26, 2019
PubMed
Summary
This summary is machine-generated.

Long non-coding RNAs (ncRNAs) associate with chromatin in the nucleus, influencing its structure and function. This review explores the connection between long ncRNAs and nuclear chromatin domains, including topologically associating domains (TADs).

More Related Videos

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.1K
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

8.8K

Related Experiment Videos

Last Updated: Jan 30, 2026

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.8K
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.1K
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

8.8K

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Large-scale transcriptome analyses reveal numerous non-coding RNAs (ncRNAs) with nuclear functions.
  • Chromatin is organized into complex, multi-layered structures including topologically associating domains (TADs).
  • ncRNAs are increasingly recognized for their roles in regulating chromatin structure and gene expression.

Purpose of the Study:

  • To review the emerging link between long non-coding RNAs (lncRNAs) and chromatin domains.
  • To highlight the role of lncRNAs in organizing nuclear architecture and gene regulation.

Main Methods:

  • Review of recent studies utilizing Hi-C technologies.
  • Analysis of findings on ncRNA association with chromatin and its regulatory proteins.
  • Integration of data on lncRNA-mediated coordination of gene transcription within chromatin domains.

Main Results:

  • TADs are mega-base self-associating chromatin domains.
  • Boundaries of TADs are often defined by CTCF and cohesin, proteins that can be recruited by ncRNAs.
  • Some ncRNAs form nuclear 'RNA clouds' that coordinate gene transcription across entire chromatin domains.

Conclusions:

  • Long non-coding RNAs play a significant role in the organization and function of nuclear chromatin domains.
  • The interaction between lncRNAs and chromatin domains is a critical mechanism for gene regulation.