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A novel thermometer-type hydrogel senor for glutathione detection
Rui Wu1, Hongguang Ge1, Cunfang Liu1
1College of Chemical and Environment Science, Shaanxi key laboratory of catalysis and application, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, PR China.
Talanta
|January 27, 2019
Summary
A novel thermometer-style visual sensor using fluorescent hydrogel enables naked-eye detection of glutathione (GSH). This innovative sensor accurately quantifies GSH in real samples, demonstrating its practical utility.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biochemistry
Background:
- Glutathione (GSH) is a crucial biomarker for oxidative stress and various diseases.
- Accurate and accessible sensing methods for GSH are vital for diagnostics.
- Stimulus-responsive hydrogels offer potential for developing novel sensors.
Purpose of the Study:
- To develop a visual sensor for glutathione (GSH) detection.
- To utilize a stimulus-responsive fluorescent hydrogel for quantitative GSH analysis.
- To demonstrate the sensor's capability for real-world sample analysis.
Main Methods:
- Synthesized a fluorescent hydrogel by crosslinking 5,6-bicarboxylic fluorescein with partly ammoniated polyacrylamide.
- Optimized experimental parameters including hydrogel particle size, buffer solution, and swelling time.
- Designed a thermometer-type visual sensor for direct volume measurement of hydrogel swelling.
Main Results:
- The hydrogel volume change directly correlated with GSH concentration within a specific range.
- The visual sensor allowed for naked-eye measurement of GSH concentration via a thermometer-like scale.
- The sensor demonstrated satisfactory agreement with High-Performance Liquid Chromatography (HPLC) results in tablet assays.
Conclusions:
- The developed thermometer-type visual sensor is effective for quantitative GSH analysis.
- Stimulus-responsive hydrogels are highly useful for intelligent sensor design.
- The thermometer-style visual sensor design is suitable for practical quantitative assays.
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