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Updated: Jan 30, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Loss of claudin-1 expression induces epithelial-mesenchymal transition through nuclear factor-κB activation in
Nae Yu Kim1, Jung-Soo Pyo2, Dong-Wook Kang2
1Department of Internal Medicine, Eulji University Hospital, Eulji University School of Medicine, Daejeon, Republic of Korea.
Objective:
The aim of this study was to elucidate the clinicopathological significance and prognostic role of loss of claudin-1 in colorectal cancer (CRC).
Methods:
The correlations between claudin-1 expression and clinicopathological characteristics, including survival rates, were assessed using immunohistochemistry on 260 archival, paraffin-embedded CRC tissues. In addition, the correlations between cludin-1 and nuclear factor-kappa B (NF-κB), epithelial-mesenchymal transition markers and tumor-infiltrating lymphocytes were investigated.
Results:
Claudin-1 expression was markedly lost in 42.7% of the 260 CRCs analyzed. Loss of claudin-1 expression significantly correlated with larger tumor size, vascular invasion, higher pT stage, and high metastatic lymph node ratio. In addition, loss of claudin-1 expression significantly correlated with NF-κB activation (P < 0.001), high SNAI (P < 0.001), and low E-cadherin (P < 0.001) expressions. Patients with high immunoscores showed significantly lower rates of claudin-1 expression loss (P = 0.020). In detail, loss of claudin-1 expression were frequently found in CRCs low CD3- and CD8-positive lymphocytes. There were significant correlations between claudin-1 expression loss and poor overall and recurrence-free survivals (P < 0.001 and P < 0.001, respectively).
Conclusion:
Taken together, our results suggest that the loss of claudin-1 expression significantly correlates with aggressive tumor behaviors, high SNAI expression, lower immunoscore, and poor prognoses.
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