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The Structure of Intermediate Filaments01:19

The Structure of Intermediate Filaments

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The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
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Parallel Resonance01:23

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The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
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Parallel Processing01:20

Parallel Processing

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The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
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Shear Diagram01:27

Shear Diagram

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In the study of beam mechanics, shear diagrams play a crucial role in understanding the distribution of shear forces along the length of a beam. Consider a beam AB that is supported at both ends and subjected to perpendicular loads.
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Shearing Stress01:19

Shearing Stress

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Shearing stress, denoted by the Greek letter tau (τ), is stress caused by forces acting transversely on an object. These forces create internal ones within the entity in the plane where the external forces are applied. The resultant of these internal forces is the shear in the section.
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Shearing Strain01:20

Shearing Strain

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The shearing strain represents a cubic element's angular change when subjected to shearing stress. This type of stress can transform a cube into an oblique parallelepiped without influencing normal strains. The cubic element experiences a significant transformation when exposed solely to shearing stress. Its shape alters from a perfect cube into a rhomboid, clearly demonstrating the effect of shearing strain. The degree of this strain is considered positive if it reduces the angle between the...
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Related Experiment Video

Updated: Jan 30, 2026

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
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Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

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Structuring of multiple parallel pectin gel filaments by applied shear.

Norihiro Kato1, Keisyu Nagayoshi1, Yuriko Takayama1

  • 1Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi 321-8585, Japan.

International Journal of Biological Macromolecules
|January 27, 2019
PubMed
Summary

Researchers created bundled micron-sized pectin gel filaments using shear-induced gelation of pectin-polyethylene glycol (PEG) assemblies. This biomimetic structure offers potential as biodegradable scaffolds for cell engineering applications.

Keywords:
Aqueous two-phase systemPectinShear-induced structuring

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Microfluidics

Background:

  • Pectin-based hydrogels are promising for biomedical applications.
  • Controlling the microarchitecture of hydrogels is crucial for function.
  • Previous methods for creating filamentous structures often require complex nozzles.

Purpose of the Study:

  • To develop a novel method for fabricating biomimetic bundled pectin gel filaments.
  • To utilize aqueous two-phase separation and microfluidics for controlled assembly.
  • To explore the potential of these structures as biodegradable scaffolds.

Main Methods:

  • Formation of pectin-polyethylene glycol (PEG) assemblies via aqueous two-phase separation.
  • Shear-induced elongation of pectin-PEG assemblies in a microfluidic device.
  • Crosslinking of pectin filaments with Ca2+ in the presence of shear-responsive PEG assemblies.
  • Utilizing PEG as a sacrificial polymer to prevent filament fusion.

Main Results:

  • Successfully formed micron-sized bundled pectin gel filaments.
  • Achieved shear-dependent elongation and controlled filament generation.
  • Demonstrated prevention of filament fusion by shear-responsive PEG assemblies.
  • Created a biomimetic bundled filamentous structure without a multi-hole nozzle.

Conclusions:

  • A novel, efficient method for creating bundled pectin gel filaments was established.
  • The developed technique leverages bio-safe polymers and microfluidics.
  • These bundled gel filaments show significant potential as biodegradable scaffolds for cell engineering.