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CRISPR-Cas9 for cancer therapy: Opportunities and challenges
Minjiang Chen1, Aiwu Mao2, Min Xu1
1Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, The Fifth Affiliated Hospital of Wenzhou Medical University, Affiliated Lishui Hospital of Zhejiang University, The Central Hospital of Zhejiang Lishui, Lishui, 323000, China.
Cancer Letters
|January 27, 2019
Summary
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 gene editing shows promise for cancer therapy by modifying genes. However, challenges like delivery and off-target effects must be addressed for clinical use.
Area of Science:
- Genetics
- Molecular Biology
- Biotechnology
Background:
- Cancer is fundamentally a genetic disease driven by accumulated mutations.
- Genome editing technologies offer novel therapeutic strategies for genetic diseases.
- CRISPR-Cas9 is a powerful tool for gene disruption and modification.
Purpose of the Study:
- To review the applications of CRISPR-Cas9 in cancer therapy.
- To highlight recent advancements in CRISPR-Cas9 for cancer treatment.
- To discuss the challenges hindering clinical translation of CRISPR-Cas9 cancer therapies.
Main Methods:
- Literature review of CRISPR-Cas9 applications in cancer research.
- Analysis of recent studies on CRISPR-Cas9-based cancer treatments.
- Discussion of technical and safety challenges in clinical settings.
Main Results:
- CRISPR-Cas9 facilitates efficient gene editing for potential cancer treatment.
- Significant progress has been made in applying CRISPR-Cas9 in various cancer models.
- Key challenges include ensuring edited cell fitness, optimizing delivery, and minimizing off-target mutations.
Conclusions:
- CRISPR-Cas9 holds immense potential for revolutionizing cancer therapy.
- Overcoming current limitations is crucial for successful clinical implementation.
- Further research is needed to refine CRISPR-Cas9 technology for safe and effective cancer treatment.
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