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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Complete genome sequence of Bacillus megaterium JX285 isolated from Camellia oleifera rhizosphere
Fang-Liang Huang1, Yang Zhang2, Lin-Ping Zhang2
1College of Life Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310012, China.
Abstract:
Bacillus megaterium strain JX285, isolated from rhizosphere red soil sample, can solubilize inorganic phosphorus, which increases the amount of available phosphorus and promotes plant growth. To investigate the mechanisms underlying phosphate solubilization, we sequenced the entire genome of B. megaterium strain JX285 (CGMCC 1.1621), which comprises a circular chromosome of 5,066,463 bp and seven plasmids of 167,030, 128,297, 60,905, 134,795, 9,598, 37,455, and 6332 bp, respectively. The whole genome sequence includes 5948 protein-coding genes, 124 tRNAs, and 29 rRNAs, and has been deposited at Genbank/EMBL/DDBJ with accession numbers CP018874-CP018881. We detected genes associated with organic acid production, which may be vital for phosphate conversion. Furthermore, phosphatase-encoding genes were also detected. The information embedded in the genome will assist in studying the mechanisms of phosphate solubilization. In conclusion, analysis of the JX285 genome will further our knowledge regarding this strain and may contribute to its biotechnological application.
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