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Flow Cytometry Purification of Mouse Meiotic Cells
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The utility of flow cytometry for potable reuse
Nicole Rockey1, Heather N Bischel2, Tamar Kohn3
1Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, USA.
Current Opinion in Biotechnology
|January 27, 2019
Summary
Real-time monitoring of virus removal in water reuse is crucial for public health. Flow virometry (FVM) offers a promising method for accurately measuring virus reduction during water treatment.
Area of Science:
- Environmental Science
- Microbiology
- Water Treatment Technology
Background:
- Protecting public health necessitates effective pathogen removal in planned water reuse.
- Viruses are a major concern in water reuse, but current real-time monitoring lacks virus measurement capabilities.
- Flow cytometry (FCM) excels at bacteria monitoring, but methods for viruses and protozoa are underdeveloped.
Purpose of the Study:
- To review advancements in flow cytometry (FCM) for microorganism quantification in water.
- To highlight the potential of flow virometry (FVM) for virus enumeration in water reuse applications.
- To propose FVM for near real-time monitoring of virus removal during water treatment.
Main Methods:
- Review of recent flow cytometry (FCM) field advancements.
- Focus on flow virometry (FVM) developments for virus enumeration.
- Application of FVM for quantifying microorganisms in water.
Main Results:
- Flow virometry (FVM) shows promise for enumerating viruses in water.
- FVM can potentially enable near real-time monitoring of virus removal.
- FVM can aid in characterizing virus removal in understudied treatment processes.
Conclusions:
- Flow virometry (FVM) is a promising technology for real-time virus monitoring in water reuse.
- FVM can enhance the validation of virus particle removal across treatment stages.
- Further studies using FVM are recommended for understudied unit processes in water treatment.
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