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Published on: December 3, 2020
HPLC-MS/MS analysis of ilimaquinone and its application in a pharmacokinetic study in rats
Heebin Son1, Keumhan Noh2, Changhyun Kang1
1College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
Abstract:
Ilimaquinone, a metabolite isolated from the marine sponge Hippiospongia metachromia, has antimicrobial, cytotoxic, anti-HIV, anti-inflammatory, and anti-cancer activities. A new quantitative analytical method for determination of ilimaquinone in rat plasma using HPLC-MS/MS was developed and validated. Ascorbic acid was added to ensure the stability of ilimaquinone in plasma. After protein precipitation using acetonitrile plus diclofenac as an internal standard, the analytes were chromatographed on a biphenyl column with a mobile phase of methanol and water (8:2, v/v, including 0.1% formic acid). This method was successfully applied in a pharmacokinetic study of ilimaquinone after oral administration in rats.
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