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Updated: Jan 30, 2026

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
Published on: September 25, 2021
An efficient induction protocol for deriving mature oligodendrocytes from human dental stem cells
Introduction:
This study examined the process of deriving mature oligodendrocytes from human dental pulp stem cells (hDPSCs) via addition of cerebrospinal fluid (CSF).
Methods:
The hDPSCs were cultured in the presence of retinoic acid and CSF. The oligodendrocytes were confirmed using immunocytochemistry for specific glial markers, namely Olig2 and MBP markers.
Results:
The differentiated oligodendrocytes were immunopositive for Olig2 and MBP markers at the end of induction phase.
Conclusion:
It is concluded that this study indicated the glial differentiation of hDPSCs in the presence of CSF and appropriate inducers, which is a usable therapeutic technique in neuroregenerative medicine (Fig. 3, Ref. 24).
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