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Oxyphor 2P: A High-Performance Probe for Deep-Tissue Longitudinal Oxygen Imaging.

Tatiana V Esipova1, Matthew J P Barrett2, Eva Erlebach3

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|January 29, 2019
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Summary

A new oxygen sensor, Oxyphor 2P, enables deeper and faster in vivo oxygen imaging using two-photon phosphorescence lifetime microscopy (2PLM). This breakthrough allows for longitudinal studies of tissue metabolism and oxygen dynamics, even after induced micro-strokes.

Keywords:
dendrimerextravascular deliveryhypoxiaoxygen imagingphosphorescenceporphyrinstroketwo-photon microscopy

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Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Physiology

Background:

  • Quantitative oxygen imaging is crucial for understanding tissue metabolism in health and disease.
  • Two-photon phosphorescence lifetime microscopy (2PLM) offers 3D oxygen measurements in vivo but is limited by probe technology.
  • Current limitations restrict imaging depth and speed, hindering broader application of 2PLM.

Purpose of the Study:

  • To develop a novel phosphorescent probe for enhanced oxygen microscopy.
  • To overcome the depth and speed limitations of existing 2PLM oxygen sensors.
  • To demonstrate the utility of the new probe for longitudinal in vivo oxygen measurements.

Main Methods:

  • Development and characterization of a new phosphorescent probe, Oxyphor 2P.
  • Application of Oxyphor 2P with two-photon phosphorescence lifetime microscopy (2PLM).
  • In vivo imaging of oxygen dynamics in mouse brain tissue following induced micro-stroke.

Main Results:

  • Oxyphor 2P enables oxygen microscopy up to 600 μm deep, twice the previous depth.
  • Imaging speed is increased by approximately 60 times compared to previous methods.
  • Longitudinal oxygen dynamics were monitored for days post-micro-stroke without direct injection into the imaged area.

Conclusions:

  • Oxyphor 2P significantly advances 2PLM capabilities for oxygen sensing in vivo.
  • The enhanced depth and speed facilitate new research into tissue metabolic states.
  • This probe opens avenues for studying oxygen dynamics in various biomedical research contexts.