Related Experiment Video

Updated: Jan 30, 2026

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
09:23

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

14.8K

CSF1R Stimulation Promotes Increased Neuroprotection by CD11c+ Microglia in EAE

Agnieszka Wlodarczyk1,2, Anouk Benmamar-Badel1,2, Oriane Cédile1

  • 1Department of Neurobiology Research, Institute for Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Frontiers in Cellular Neuroscience
|January 29, 2019
PubMed

Insights

Stimulating Colony Stimulating Factor-1 receptor (CSF1R) expands CD11c+ microglia, reducing central nervous system inflammation and demyelination. This suggests a new therapeutic target for neuroinflammatory diseases like multiple sclerosis.

Area of Science:

  • Neuroimmunology
  • Cellular Biology

Background:

  • Microglia, the central nervous system's immune cells, are vital for neurodevelopment and myelination.
  • A specific subset, CD11c+ microglia, crucial for myelination, expands during neuroinflammation but its triggers are unknown.
  • These CD11c+ microglia are implicated in protective roles in central nervous system (CNS) diseases.

Purpose of the Study:

  • To identify the molecular triggers for CD11c+ microglia expansion.
  • To investigate the therapeutic potential of modulating microglial populations in neuroinflammatory conditions.

Main Methods:

  • Stimulation of Colony Stimulating Factor-1 receptor (CSF1R) using its ligands, CSF1 and IL-34.
  • Induction of CD11c+ microglia expansion and assessment of experimental autoimmune encephalomyelitis (EAE) symptoms.
  • Investigating the role of chemokine CCL2 and its receptor CCR2 in microglial expansion.

Main Results:

  • CSF1R stimulation by CSF1 or IL-34 induced CD11c+ microglia expansion.
  • This expansion ameliorated EAE symptoms and reduced demyelination.
  • CCL2 induction by CSF1R ligands and experimental CCL2 overexpression led to CD11c+ microglia increase, suggesting a positive feedback loop involving CSF1R and CCL2.

Conclusions:

  • CSF1R signaling is a key regulator of CD11c+ microglia expansion.
  • Modulating CSF1R offers a potential therapeutic strategy for neuroinflammatory diseases.
  • A positive feedback loop between CSF1R and CCL2 influences microglial responses in the CNS.
Keywords:
CCL2CD11cCSF1CSF1REAEIL-34microglia

More Related Videos

Strategies for Study of Neuroprotection from Cold-preconditioning16:27

Strategies for Study of Neuroprotection from Cold-preconditioning

Published on: September 2, 2010

15.3K
Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium12:00

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Published on: March 9, 2018

17.0K

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

3.9K
Increasing Function01:18

Increasing Function

An increasing function exhibits a rise in output values as input values increase. This behavior is depicted graphically as a curve or line that slopes upward from left to right. Such a function satisfies the condition that if x1 < x2, then f(x1) < f(x2), indicating that the function values grow with increasing inputs. This concept is fundamental in understanding growth trends across various domains, such as population dynamics, financial investments, or resource consumption.The...
391
Increased Body Temperature01:25

Increased Body Temperature

A body temperature above  38°C  (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
7.5K
Increased pulse rate01:17

Increased pulse rate

Tachycardia is a condition marked by an abnormally fast or irregular heart rate, surpassing the typical resting rate. In adults, tachycardia is characterized by a pulse rate ranging from 100 to 180 beats per minute. The increased heart rate can result in inadequate blood flow to various body parts, ultimately diminishing the oxygen supply to organs and tissues.
Many factors can elevate the risk of developing tachycardia. These include advanced age, a family history of arrhythmias, and an...
1.2K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.4K