Neuropeptide Y and vasoactive intestinal peptide coexist in rat thyroid nerve fibers emanating from the thyroid...

Neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) occur in nerve fibers around blood vessels and between follicles in the thyroid gland of the mouse and rat. VIP-immunoreactive fibers are numerous, while NPY-immunoreactive fibers are fewer. Most of the latter fibers contain noradrenaline (NA) as well as NPY, while a subpopulation was found to contain VIP instead of NA. We have determined the origins of rat thyroid nerve fibers containing NPY, VIP or NPY/VIP by investigating 3 conceivable sources, i.e. the superior cervical ganglion, the nodose ganglion and the thyroid ganglion. Chemical sympathectomy or removal of the superior cervical ganglion did not affect the frequency of VIP-immunoreactive fibers but eliminated most of the NPY-immunoreactive fibers as well as all NA-containing nerve fibers (recognized by antibodies to dopamine-beta-hydroxylase). The NPY-immunoreactive fibers that remained after sympathectomy occurred around blood vessels and between follicles and contained VIP. Cervical vagotomy (removal of the nodose ganglion including the adjacent vagus) did not overtly affect the frequency of NPY/VIP-, VIP-, or NPY/NA-containing fibers in the thyroid. In contrast, extirpation of the thyroid ganglion, which is situated immediately outside the thyroid capsule, greatly reduced the number of VIP- and NPY/VIP-containing fibers in the rat thyroid. On the whole, the results of radioimmunoassay of NPY and VIP agreed well with the immunocytochemical findings. High performance liquid chromatography confirmed the identity of NPY and VIP. The present findings suggest the existence in the rat thyroid of one NPY-containing nerve fiber population that harbours NA and emanates from the superior cervical ganglion; one NPY-containing fiber population that is non-adrenergic, harbours VIP and originates in the thyroid ganglion; and a second VIP-containing fiber population that is devoid of NPY and appears to derive from the thyroid ganglion.