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Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
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Simple Detection of Hepatitis B Virus in Using Loop-Mediated Isothermal Amplification Method
Hua-Wei Chen1, Tatyana Belinskaya1, Zhiwen Zhang1
1Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD.
Military Medicine
|January 29, 2019
Summary
A new loop-mediated isothermal amplification (LAMP) assay can detect Hepatitis B virus (HBV) in blood products. This rapid screening method improves blood safety in resource-limited and deployed settings.
Area of Science:
- Medical Diagnostics
- Virology
- Public Health
Background:
- Hepatitis B virus (HBV) is endemic in regions where US military and civilian personnel deploy.
- Transfusions with locally collected blood are common in emergencies, posing a risk of HBV transmission.
- Current screening methods for fresh blood in deployed settings are inadequate.
Purpose of the Study:
- To develop a sensitive and rapid assay for detecting HBV in blood products.
- To provide a screening tool for fresh blood transfusions in resource-limited and deployed environments.
Main Methods:
- Developed a loop-mediated isothermal amplification (LAMP) assay targeting the pre-Surface/Surface antigen gene of HBV.
- Optimized heat treatment for plasma specimens without DNA extraction (125°C for 10 min).
- Detected amplicon using a fluorescence tube scanner or immune-chromatography test strip.
Main Results:
- The LAMP assay detected as few as 10 copies of HBV DNA per sample.
- The assay correctly identified HBV in 107 out of 127 (84%) samples.
- Optimized conditions allowed for rapid screening without prior DNA extraction.
Conclusions:
- The developed LAMP assay offers a sensitive and efficient method for HBV detection.
- This assay has significant potential for improving blood safety in resource-limited and deployed settings.
- The assay provides a crucial tool for preventing HBV transmission through blood transfusions in high-risk areas.
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