Production of feather oligopeptides by a newly isolated bacterium Pseudomonas otitis H11

Oligopeptides usually have high nutritive value and multiple physiological functions. To achieve the highly efficient utilization of feather waste, a feather-degrading bacterium, Pseudomonas otitis, was isolated and used for production of feather oligopeptides. The production potential and characteristics of the produced oligopeptides by H11 were also investigated. The results demonstrated that the optimal initial pH, temperature, fermentation time, and sterilization conditions were 11, 40°C, 24 h, and 121°C for 20 min, respectively. After 24 h of fermentation under the optimal conditions, the feathers were almost completely degraded. Correspondingly, 35.37% oligopeptides (accounting for 69.70% of the total soluble peptides) and varieties of essential amino acids (valine, isoleucine, leucine, phenylalanine, methionine, threonine, and lysine) were obtained. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the produced oligopeptides were mainly low molecular weight (below 1600 Da) and rich in branched-chain amino acids. Also, the oligopeptide-enriched hydrolysate displayed good antioxidant activity with 83% 2,2-diphenyl-1-picrylhydrazyl (DPPH•) scavenging ability and 53% superoxide anion (O2•-) scavenging activity. This study demonstrated that the hydrolysate of feathers was abundant in oligopeptide fractions with 5-10amino acid residues and possessed good antioxidant activity. This oligopeptide-enriched hydrolysate could be used as a functional feed supplement and as a source for functional oligopeptide extraction.