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Somatostatin Receptor 2 Expression in Canine Meningioma
G Foiani1, G Guelfi1, E Chiaradia1
1Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia, Italy.
Journal of Comparative Pathology
|January 30, 2019
Summary
Canine meningiomas express somatostatin receptor subtype 2 (SSTR2), a potential target for new treatments. This study found SSTR2 is present in most tumors and cell cultures, offering hope for medical therapies.
Area of Science:
- Veterinary Oncology
- Molecular Biology
- Neuropeptide Signaling
Background:
- Somatostatin (SST) and its receptors (SSTRs) regulate cell proliferation in human tumors.
- SSTR2 is frequently found in human meningiomas and is a target for medical treatments.
- Canine meningiomas are common brain tumors in dogs, often requiring advanced treatment strategies.
Purpose of the Study:
- To investigate the expression of somatostatin receptor subtype 2 (SSTR2) in canine meningiomas.
- To determine if SSTR2 expression correlates with tumor characteristics like subtype and grade.
- To assess the potential of SSTR2 as a therapeutic target in canine meningiomas.
Main Methods:
- Immunohistochemistry (IHC) and reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) were used to evaluate SSTR2 expression in 46 canine meningiomas.
- Immunocytochemistry, western blotting, and RT-qPCR were performed on primary cell cultures from two canine meningiomas.
- Tumor histological subtype and grade were analyzed in relation to SSTR2 expression levels.
Main Results:
- SSTR2 was detected by IHC in 81% (17/21) and by RT-qPCR in 56% (14/25) of canine meningiomas.
- SSTR2 expression levels did not significantly correlate with tumor subtype or grade, although meningothelial meningiomas showed high expression.
- A trend towards reduced SSTR2 expression in high-grade meningiomas was observed.
- SSTR2 expression was confirmed in canine meningioma cell cultures, with approximately 90% showing expression.
Conclusions:
- Canine meningiomas express SSTR2, both in vivo and in vitro.
- SSTR2 expression is maintained in cultured canine meningioma cells.
- The presence of SSTR2 in canine meningiomas suggests potential for novel medical treatment strategies, particularly for unresectable or recurrent tumors.
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