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Updated: Jan 30, 2026

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

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Evaluation of bacterial inactivation in random donor platelets and single-donor apheresis platelets by the INTERCEPT

Raj Nath Makroo1, Raman Sardana2, Leena Mediratta2

  • 1Department of Transfusion Medicine, Indraprastha Apollo Hospitals, New Delhi, India.

Asian Journal of Transfusion Science
|January 30, 2019
PubMed
Abstract

Insights

The INTERCEPT system effectively inactivates bacteria in platelet components, significantly reducing contamination risks. This pathogen inactivation method offers a vital safety measure against bacterial sepsis from transfused platelets.

Area of Science:

  • Transfusion Medicine
  • Microbiology
  • Biotechnology

Background:

  • Bacterial contamination of blood products, particularly platelets, poses a significant risk of sepsis.
  • Platelets are highly susceptible to contamination due to collection and storage methods.
  • Despite interventions, transfusion-transmitted bacterial sepsis remains a common concern.

Purpose of the Study:

  • To evaluate the efficacy of the INTERCEPT (Amotosalen/UVA) pathogen inactivation system.
  • To assess the system's ability to inactivate bacteria in deliberately contaminated platelet units.

Main Methods:

  • Single-donor platelets (SDP) and random-donor platelets (RDP) were intentionally contaminated with Staphylococcus aureus and Escherichia coli.
  • Bacterial concentrations were quantified before and after treatment with the INTERCEPT system.
  • Platelet units underwent pathogen inactivation using the INTERCEPT Blood System for platelets.

Main Results:

  • No bacterial growth was detected in post-illumination test samples.
  • A >6 log10 reduction in Staphylococcus aureus in SDP units was achieved.
  • A >6 log10 reduction in Escherichia coli in RDP units was achieved.

Conclusions:

  • The INTERCEPT system demonstrated high effectiveness in bacterial inactivation in platelet components.
  • Implementing the INTERCEPT system can mitigate the risk of bacterial contamination in platelet transfusions.
  • This technology offers a promising strategy to enhance blood product safety.
Keywords:
Amotosalenbacterial contaminationpathogen inactivationrandom donor plateletssepsissingle-donor plateletstransfusionultraviolet A

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