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Gene manipulation by homologous recombination in Escherichia coli
1Department of Biology, Emory University, Atlanta, GA 30322.
DNA (Mary Ann Liebert, Inc.)
|December 1, 1988
Abstract:
The use of homologous recombination in Escherichia coli is described as a tool for DNA manipulation. The utility of the method is illustrated by the addition of 3'-flanking sequences to a dhfr minigene by plasmid-phage recombination involving a supF-containing dhfr minigene plasmid and a lambda Charon4A phage containing the 3' end of the dhfr gene. In addition, other uses of both plasmid-phage and phage-phage recombination in gene manipulation are described.