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Updated: Jan 30, 2026

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Published on: June 6, 2025
Grafting as a Tool for Study of Phototropism in Phycomyces
Atsushi Miyazaki1, Tamotsu Ootaki2
1Department of Biological Sciences, Faculty of Science and Engineering, Ishinomaki Senshu University, Ishinomaki, Miyagi, Japan. miyazaki@isenshu-u.ac.jp.
Abstract:
Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.
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